Source
Rat Tail Tendon

Quality
Purity = 90% by SDS-PAGE
Tested for: ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Forms firm gel up to a dilution of 1:10; further dilution will decrease rigidity of gel
Tested and found negative for bacteria, fungi, and mycoplasma

Formulation
Liquid in 0.02 N acetic acid, concentration range 3-4 mg/mL

Preparation and Storage
Stable for at least three months at 2° to 8°C. Do not freeze.

Handling
Effective as a gel or thin coating at a recommended concentration of 5-10 µg/cm2 of growth surface depending on cell type