详细描述

Exosome是一种40-100nm的膜被囊泡，由大部分类型的细胞在活体内或者在活体外分泌。外吐小体发现于血液、羊水、恶性腹水，和含有因分泌肿瘤的不同而分为不同亚群的mRNAs中。SBI的Exoquick外吐小体沉淀物试剂可以使mRNAs生物标记物探索简单、可靠且定量。使用Exoquick增浓了环状外泌体mRNAs且使用SBI’s SeraMir™ 或QuantiMir™ qPCR arrays能准确地描述它们。

产品特点：

• 无耗时超速离心
• 价格低于昂贵的
• 比其他方法更有效
• 使用尽可能少的100ul血清或生物液的量

ExoQuick™ 试剂盒在室温下或蓝色冰下运输且一旦收到需在+4°C存储。自收到日起，若正确存储试剂盒可稳定一年。反应用量基于250ul血清。各种生物液的沉淀外吐小体的例子见于下表。

ExoQuick™ is a proprietary polymer that gently precipitates exosomes and microvesicles between 30 and 200 nm in size from serum or ascites fluid. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your intact exosomes will be in the pellet, ready for resuspension in an appropriate solution. 

List of Components 

Item                                                        Catalog #      Volume         Reactions

 ExoQuick™ Exosome Precipitation Solution   EXOQ20A-1    20 ml           300 reactions 

ExoQuick™ Exosome Precipitation Solution      EXOQ5A-1     5 ml            75 reactions 

Storage 

The ExoQuick kits are shipped at room temperature, blue ice or dry ice and should be stored at +4°C or room temperature (+25°C ) upon receipt. Properly stored kits are stable for 1 year from the date received. 

General Information 

The reaction size is based on using 250 µl of serum for exosome isolation. Examples of precipitating exosomes from various biofluids can be seen in the Table below. These volumes can be scaled up or down accordingly. We recommend a minimum starting sample volume of at least 100 µl. 

To isolate exosomes from plasma, we recommend using the ExoQuick™ Plasma Prep and Exosome Precipitation Kit (Cat# EXOQ5TMA-1). Plasma contains fibrin which will precipitate along with ExoQuick™ causing an insoluble pellet to form. The ExoQuick Plasma Prep and Exosome Precipitation kit contains reagents to help dissolve the fibrin, thus increasing the yield of exosomes precipitated.