Catalog Number

553073

Brand

BD Pharmingen™

Name

CD25

Alias

IL-2 Receptor α chain, p55

Concentration

1.0 mg/ml

Size

0.5 mg

Regulatory Status

 RUO

Clone

3C7

Format

NA/LE

Isotype

IgG_2b, κ

Immunogen

IL-2-dependent BALB/c mouse cell line

Strain Host

LEW

Reactivity

QC Testing : Mouse

Applications

Blocking	- Reported
Flow cytometry	- Routinely Tested
Immunohistochemistry-formalin (antigen retrieval required)	- Not Recommended
Immunohistochemistry-frozen	- Reported
Immunohistochemistry-zinc-fixed	- Reported
Neutralization	- Routinely Tested

Clone - Description

3C7 reacts with the 55 kDa low affinity (a chain, p55, CD25) IL-2 receptor subunit which is expressed by activated T and B lymphocytes from all mouse strains tested. The IL-2 receptor a chain (IL-2Ra) by itself is not a signaling receptor. However, the IL-2 receptor a chain can combine with IL-2 receptor b chains and g_c chains to form high affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express CD25. CD25 is transiently expressed at a low level during normal B-cell development at the Pre-B or Pre-B-II stage in the bone marrow, and it is expressed at a higher level during a very early stage of T cell development in fetal and adult thymus. The 3C7 antibody recognizes a CD25 epitope which is distinct from that recognized by the 7D4 antibody. The 3C7 antibody blocks the binding of IL-2 to CD25.¹

Clone - Application

Flow Cytometry: Purified and PE- 3C7 are useful for immunofluorescent staining and flow cytometry.
Neutralization: Purified NA/LE™ 3C7 is useful for neutralization.