Human CD40L ELISA Kit;人CD40配体ELISA试剂盒
详细描述
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 4pg/ml
Assay Range 8.2-6000pg/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene ID 959
Swissprot ID P29965
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 94 | 89-102 |
| Plasma | 96 | 88-107 |
| Cell culture media | 91 | 86-105 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 89 |
| 1:4 | 92 | |
| 1:8 | 90 | |
| Plasma | 1:2 | 91 |
| 1:4 | 96 | |
| 1:8 | 94 | |
| Cell culture media | 1:2 | 92 |
| 1:4 | 93 | |
| 1:8 | 101 |
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Product Summary
The Bioaim Human CD40L ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD40L in human serum, plasma and cell culture media. An antibody specific for human CD40L was coated on a 96-well plate. Standards and samples are added to the wells and any CD40L present binds to the immobilized antibody. The wells are washed and biotinylated anti-human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD40L present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Background
CD40 Ligand, also known as TNFSF5, CD154, TRAP, and gp39, is a trimeric protein that is expressed in transmembrane and soluble forms. It is expressed by many immune cell types as well as by endothelial and smooth muscle cells of the vasculature. It binds to CD40 on antigen presenting cells, platelets, and endothelial and epithelial cells, leading to enhanced B cell activation and T cell dependent humoral immune responses. CD40 Ligand dysregulation contributes to immune pathology in AIDS, atherosclerosis, atherothrombosis, and restenosis.
Regulatory Status RUO
