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Recombinant Mouse 5'-Nucleotidase/CD73 Protein, CF (10 UG)
详细描述
Source:Chinese Hamster Ovary cell line, CHO-derived, Trp29-Lys549, with a C-terminal 6-His tag
Accession #:NP_035981
N-terminal Sequence Analysis:Trp29
Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level:
Predicted Molecular Mass:59 kDa
SDS-PAGE:60 kDa, reducing conditions
Activity:Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
Formulation:Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol. See Certificate of Analysis for details.
Molecule Information:
5'-Nucleotidase/CD73
Aliases:
5-NT; CD73; E5NT; eNT; NT5E; NTE
Entrez Gene IDs:
4907 (Human); 23959 (Mouse); 58813 (Rat)
Background:
5'-Nucleotidase/CD73
CD73, an ecto-5’-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that forms a disulfide-linked homodimer. The 5’-nucleotidase activity of CD73 converts extracellular nucleoside 5’ monophosphates to nucleosides. It is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. Most cell types express CD73.
CD73, an ecto-5’-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that forms a disulfide-linked homodimer. The 5’-nucleotidase activity of CD73 converts extracellular nucleoside 5’ monophosphates to nucleosides. It is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. Most cell types express CD73.
