Description | CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. |
Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
References | 1. Personal communication (IP) 2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block) 3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. 4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block) 5. Clements JL, et al. 2006. J. Immunol. 177:905. 6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed 7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed 8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed 9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed 10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed 11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed 12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed 13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed 14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed 15. Dutow P, et al. 2014. J Infect Dis. PubMed 16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed 17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed 18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed 19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed 20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed 21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed 22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed 23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block) |