Compatible ends
Mvn I ends are compatible with any blunt end.

Isoschizomers
Mvn I is an isoschizomer of Acc II, BstU I, FnuD II, Tha I.

Methylation sensitivity
Mvn I is inhibited by the presence of 5-methylcytosine at the site indicated (*) on the recognition sequence.

Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:

Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 30%. The PCR mix contained λDNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl₂, 200 μM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.

Incubation temperature
+37°C

Unit definition
One unit is the enzyme activity that completely cleaves 1 μg  λDNA in 1 hour at +37°C in a total volume of 25 μl SuRE/Cut Buffer B. 1 µg pBR322 DNA is digested completely by approximately 1 unit of Mvn I.

Heat inactivation
There is no information about heat inactivation available.

Number of cleavage sites on different DNAs

Quality

Absence of nonspecific endonuclease activities
1 μg λDNA is incubated for 16 hours in 50 μl SuRE/Cut Buffer M with an excess of Mvn I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.

Absence of exonuclease activity
Approximately 5 μg [³H] labeled calf thymus DNA are incubated with 3 μl Mvn I for 4 hours at +37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl₂, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.

Typical ligation and recutting assay
Mvn I fragments obtained by complete digestion of 1 μg λDNA are ligated for 16 hours at +25°C with 1 U T4 DNA Ligase in 10 μl buffer that contains 66 mM Tris-HCl, 5 mM MgCl₂, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with  Mvn I (yielding the typical pattern of  λ × Mvn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.