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HP Plasmid Isolation Kit, 250
详细描述
The High Pure Plasmid Isolation Kit isolates purified plasmid DNA in small quantities using the alkaline lysis method, a commonly used method that generates highly purified plasmid DNA from E. coli, free of RNA contamination.
- Quickly purify up to 24 plasmid samples in <30 minutes.
- Minimize DNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
- Improve reliability and reproducibility in downstream applications with a kit that removes RNA and other impurities that cause plasmid DNA to behave unpredictably.
- Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.
Contents
- Suspension Buffer
- RNase A, dry powder
- Lysis Buffer
- Binding Buffer
- Wash Buffer I
- Wash Buffer II
- Elution Buffer
- High Pure Spin Filter Tubes (containing glass fiber fleece)
- Collection Tubes
The High Pure Plasmid Isolation Kit prepares up to 15 μg purified plasmid DNA from bacterial cultures, that can be used directly in most molecular biology applications:
- PCR
- Sequencing (see Figure 1)
- Cloning
- In vitro transcription
- Restriction enzyme digests
- Random primed labeling
