Loading
b-Gal Reporter Gene Assay, che
详细描述
- Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts
- High dynamic measuring range: Linear range over four to five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
- Safe: No radioactive isotopes are used
- Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
- Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control
Contents
- β-Gal Substrate, 100x concentrated, Galacton Plus
- Assay Buffer
- Enhancer, Emerald II
- Initiation Solution
- Protease Inhibitors
- Lysis Buffer, 5x concentrated
- Positive Control, β-galactosidase (E. coli), lyophilizate
The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid that bears the β-Gal-encoding lacZ gene as a reporter.
