FluoroBand 1 KB (0.25-10 kb) Fluorescent DNA Ladder

500 µl

Description

The DM3160 FluoroBand 1KB (0.25-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct loading on gel. The DNA ladder DM3160 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.

Source

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range

250 ~ 10,000 bp

Concentration

50 µg/ 500 µl

Recommended loading volume

5 µl/ well

Storage

Protected from light

Room temperature  ≥ 6 months

4°C ≥ 12 months

-20°C ≥ 36 months

DM3160 MSDS DM3160 Product Information