Overview

• Product nameAnti-FSH Receptor antibodySee all FSH Receptor primary antibodies ...
• Description
  Rabbit polyclonal to FSH Receptor
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Guinea pig, Pig, Macaque Monkey
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human FSH Receptor.

  Read Abcam's proprietary immunogen policy

• Positive control
  • This antibody gave a positive signal in Rat Ovary tissue lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • GPCR
  • More GPCR

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

  • Neuroscience
  • Endocrine system
  • Gonadotrophic axis

Applications

Target

• FunctionReceptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
• Tissue specificitySertoli cells and ovarian granulosa cells.
• Involvement in diseaseDefects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
  Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis.
• Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
  Contains 9 LRR (leucine-rich) repeats.
  Contains 1 LRRNT domain.
• Post-translational
  modificationsN-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.
• Cellular localizationCell membrane.

• Target information above from: UniProt accession P23945 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 2492 Human
  • Entrez Gene: 397679 Pig
  • Entrez Gene: 25449 Rat
  • Omim: 136435 Human
  • SwissProt: P23945 Human
  • SwissProt: P49059 Pig
  • SwissProt: P20395 Rat
  • Unigene: 1428 Human

  • Unigene: 162843 Rat

  see all
• Alternative names
  • Follicle stimulating hormone receptor antibody
  • Follicle stimulating hormone receptor isoform 1 antibody
  • Follicle-stimulating hormone receptor antibody

  • Follitropin receptor antibody
  • FSH receptor antibody
  • FSH-R antibody
  • Fshr antibody
  • FSHR_HUMAN antibody
  • FSHRO antibody
  • LGR1 antibody
  • MGC141667 antibody
  • MGC141668 antibody
  • ODG1 antibody
  • ovarian dysgenesis 1 antibody

  see all

Anti-FSH Receptor antibody images

• 

  Western blot - Anti-FSH Receptor antibody (ab103874)
  Anti-FSH Receptor antibody (ab103874) at 1 µg/ml + Ovary (Rat) Tissue Lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 78 kDa
  Observed band size : 78 kDa
  Additional bands at : 14 kDa,230 kDa,28 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-FSH Receptor antibody (ab103874)
  ICC/IF image of ab103874 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103874, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.