Overview

• Product nameGoat Anti-Rabbit IgG H&L (Alexa Fluor® 594)See all IgG secondary antibodies ...
• Description
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594)
• Target speciesRabbit
• Tested applicationsICC/IF, ELISA, IHC-P, Flow Cyt more details
• ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituents: PBS, 30% Glycerol, 1% BSA
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
• Clonality Polyclonal
• IsotypeIgG
• General notes

  The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

• Research Areas
  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

  • Secondary antibodies
  • anti-Rabbit
  • IgG
  • Fluorophore
  • Alexa Fluor® 594

Applications

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) images

• 

  Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) (ab150080)
  ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150080 Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
  HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150080 Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)