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胰酶Trypsin-EDTA (0.25 %) in HBSS (1x), with phenol red   

详细描述

胰酶Trypsin-EDTA (0.25 %) in HBSS (1x), with phenol red

Trypsin-EDTA solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.

胰蛋白酶-EDTA 溶液提取自猪胰腺,适用于多种细胞类型的消化和传代。多样化的配方可满足众多应用需求。

Storage:≤-15°C
Sterility:Sterile-filtered





产品描述
胰蛋白酶-EDTA 溶液用于将贴壁细胞从培养表面解离下来。其成分包含源自猪胰腺的天然胰蛋白酶和 EDTA。
溶液中添加的酚红可作为 pH 指示剂,以便于调整至最佳的细胞培养条件。
从基质上消化细胞所需的胰蛋白酶浓度主要取决于细胞类型和培养时间。建议尝试不同配方,以确定最适合特定应用场景的产品。
Description
Trypsin-EDTA solutions are used for the detachment of adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA.

The addition of phenol red serves as a pH indicator to adjust to the perfect cell culture conditions.

The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

引用文献:

TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring gene

E Kabirova, A Ryzhkova, V Lukyanchikova… - Nature …, 2024 - nature.com
… separated, rinsed in 10x penicillin/streptomycin solution (Capricorn Scientific, PS-B), and
incubated overnight in 0.25% trypsin/DMEM (Capricorn Scientific, TRY-2B). Next day, blood, …

Naturally derived 3-aminoquinuclidine salts as new promising therapeutic agents

D Crnčević, A Ramić, AR Kastelic, R Odžak, L Krce… - Scientific reports, 2024 - nature.com

… , Capricorn Scientific) and a 1% mixture of penicillin and streptomycin (Pen/Strep, Capricorn

Scientific). … -EDTA (0.05%) solution (Capricorn Scientific) and gently resuspended in DMEM …

[HTML] In Situ Electrosynthesis of Hyaluronic Acid Doped Polypyrrole on Polyvinyl Alcohol/Chitosan Nanofibers as a Cellular Scaffold

RL Quiroz-Oregón, A Pérez-Nava… - … of Composites Science, 2026 - mdpi.com
… Cells were trypsinized with trypsin–EDTA (0.25%, Capricorn Scientific). An indirect in
vitro assay using NIH-3T3 cells was set up to evaluate the cytotoxicity/viability of the post-…

[HTML] A single-cell Raman spectroscopy analysis of bone marrow mesenchymal stem/stromal cells to identify inter-individual diversity

T Kukolj, J Lazarević, A Borojević, U Ralević… - International Journal of …, 2022 - mdpi.com
… After reaching 80–90% of confluence, adherent BM-MSCs were passaged using 0.25%
Trypsin/EDTA solution (Capricorn-Scientific) and replated at concentration of 1 × 10 4 cells/cm 2 …

Pharmacological targeting of netrin-1 inhibits EMT in cancer

J Lengrand, I Pastushenko, S Vanuytven, Y Song… - Nature, 2023 - nature.com
… 1×) and detached from the cell culture plate with trypsin (Capricorn Scientific, TRY-2B10). …
2000 (Thermo Fisher Scientific) using Opti-medium (Capricorn Scientific). The cell line of …

Endoplasmin regulates differentiation of tonsil-derived mesenchymal stem cells into chondrocytes through ERK signaling

HR Kim, H Choi, SY Park, YC Song, JH Kim… - BMB …, 2022 - pmc.ncbi.nlm.nih.gov
… The cells were detached by 0.25% Trypsin-EDTA (Capricorn Scientific) and then … protease
inhibitors and phosphatase inhibitors (Thermo Scientific TM ). Protein concentrations were …

[HTML] Antibacterial Potential and Cytotoxicity Assessment of Zinc-Based Ternary Deep Eutectic Solvents: Towards Innovative Applications in Dental Medicine

J Filipović Tričković, N Zdolšek, S Brković, F Veljković… - Processes, 2025 - mdpi.com
… Upon the treatment in the same manner as for the XTT assay, the cells were detached from
the wells using trypsin-EDTA (Capricorn Scientific, Ebsdorfergrund, Germany) and blocked …

A Novel Hollow Fiber Infection Model (HFIM) for Antiviral PK/PD studies of CMV infection

LM Sudarsono, SAM Wenker, X Liu, J Brink… - bioRxiv, 2026 - biorxiv.org
… Trypsin (Capricorn Scientific) was used for passaging and collection of adherent cells.
Human cytomegalovirus (HCMV) Towne (ATCC ® VR-977 ™) and the Merlin strains were used …


Comparative RNA-Seq Analysis of Colon Spheroids and Patient-derived Tissues Identifies Non-Canonical Transcript Isoforms of Protein-Coding Genes Implicated in …

T Babic, B Banovic Djeri, D Pavlovic… - Cancer …, 2025 - journals.sagepub.com
… ; Capricorn Scientific, Germany) and 1% antibiotic/antimycotic solution (Capricorn Scientific,
… were detached using 1× trypsin/EDTA (Capricorn Scientific, Germany) and counted with a …

Zinc oxide nanoparticles as diagnostic tool for cancer cells

S Ibraheem, AA Kadhim, KA Kadhim… - International Journal …, 2022 - Wiley Online Library

… Dulbecco’s Modified Eagle’s Medium (DMEM) (Usbiological, USA), fetal bovine serum (FBS),

penicillin, streptomycin, and trypsin-EDTA were purchased from (Capricorn- Scientific, …


[HTML] Effect of the application of a hydrogen peroxide home bleaching agent on the cytotoxicity of different CAD-CAM restorative materials

CS Ozverel, S Kurtulmus-Yilmaz - Dental and Medical Problems, 2023 - dmp.umw.edu.pl
… After reaching 80% confluence, the cells were trypsinized with trypsin-EDTA (Capricorn
Scientific, Ebsdorfergrund, Germany) for 10 min at 37C and 5% CO 2 before being used in the

Transcript CD81‐215 may be a long noncoding RNA of stromal origin with tumor‐promoting role in colon cancer

E Jovanovic, T Babic, S Dragicevic… - Cell Biochemistry …, 2023 - Wiley Online Library

… For the generation of spheroids, adherent cells were detached with 1x trypsin/EDTA (Capricorn

Scientific) and counted using a standard hemocytometer. For each cell line, …


[HTML] The role of doxycycline and IL-17 in regenerative potential of periodontal ligament stem cells: Implications in periodontitis

I Okić Đorđević, T Kukolj, M Živanović, S Momčilović… - Biomolecules, 2023 - mdpi.com
… ; Capricorn Scientific, Ebsdorfergrund, Germany) supplemented with 10% fetal bovine serum
(FBS; Capricorn Scientific… % Tripsyn/EDTA (Capricorn Scientific, Ebsdorfergrund, Germany) …
  • 目录号:TRY-3B
  • 品牌:Capricorn Scientific
  • 规格:100 ml
  • 目录价:¥180.00
  • 市场价格:¥180.00
  • 会员价格:¥180.00
  • 数量:

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