详细描述

HEPES Buffer Solution (1M) is a biological buffer used in cell culture media. Due to its stability, it is suitable for many different cell culture systems.

HEPES缓冲液（1M）是一种用于细胞培养基的生物学缓冲剂。由于其稳定性优异，适用于多种不同的细胞培养体系。

Cat-Cat-No:HEP-B

Volume:100 ml
Storage:+2°C to +8°C

Sterility:Sterile-filtered

产品描述
HEPES（即N-2-羟乙基哌嗪-N-2-乙磺酸）是一种浓度为1 M的缓冲液。作为一种两性离子有机化学缓冲剂，它被广泛应用于细胞培养基中。该产品能将pH值稳定在6.7至8.6的范围内。当细胞培养需要在CO₂培养箱外进行长时间操作时，添加10至25 mM的HEPES能提供额外的缓冲能力。

我们的HEPES缓冲液具有膜不可渗透性、对生化反应影响小以及化学与酶稳定性高的特点，因此适用于多种细胞培养系统。该溶液采用细胞培养级水配制而成。

Description
HEPES is a buffer solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid. HEPES is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when a cell culture requires extended periods of manipulation outside of a CO2 incubator.

Our HEPES buffer solution is suitable for many cell culture systems because it is membrane impermeable, has a limited effect on biochemical reactions, and is chemically and enzymatically stable. The solution is prepared in cell culture grade water.

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… mM HEPES (Capricorn Scientific), and 0.1 mM nonessential amino acids (Capricorn Scientific).

… ; Capricorn Scientific) supplemented with 10% FBS (Sigma Aldrich), 2mM L-Glutamine (…

Preclinical Evaluation of 99mTc-Labeled LHRH Analog as Cancer Receptor Imaging

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… (Capricorn … (Capricorn Scienti c) supplemented with FBS (Capricorn Scienti c) at 10%

nal concentration, 100 U/mL penicillin,100 ug/mL streptomycin and 100 ug/mL HEPES (Capricorn …

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… at a multiplicity of infection 0.01 in DMEM (Capricorn, Germany) supplemented with 1 μg/mL …

supplemented with AA solution, 25 mM HEPES (all from Capricorn, Germany) and 50 μM 2-…

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… The seminal samples were thoroughly washed by centrifugation with HEPES Buffer Solution

(Capricorn Scientific GmbH, Germany), and the pelts were kept at -80C until needed (28). …

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