HEPES缓冲液 Buffer Solution (1 M)   
详细描述
HEPES Buffer Solution (1M) is a biological buffer used in cell culture media. Due to its stability, it is suitable for many different cell culture systems.
HEPES缓冲液(1M)是一种用于细胞培养基的生物学缓冲剂。由于其稳定性优异,适用于多种不同的细胞培养体系。
Cat-Cat-No:HEP-B
Volume:100 mlStorage:+2°C to +8°C
Sterility:Sterile-filtered
产品描述
HEPES(即N-2-羟乙基哌嗪-N-2-乙磺酸)是一种浓度为1 M的缓冲液。作为一种两性离子有机化学缓冲剂,它被广泛应用于细胞培养基中。该产品能将pH值稳定在6.7至8.6的范围内。当细胞培养需要在CO₂培养箱外进行长时间操作时,添加10至25 mM的HEPES能提供额外的缓冲能力。
我们的HEPES缓冲液具有膜不可渗透性、对生化反应影响小以及化学与酶稳定性高的特点,因此适用于多种细胞培养系统。该溶液采用细胞培养级水配制而成。
HEPES is a buffer solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid. HEPES is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when a cell culture requires extended periods of manipulation outside of a CO2 incubator.
Our HEPES buffer solution is suitable for many cell culture systems because it is membrane impermeable, has a limited effect on biochemical reactions, and is chemically and enzymatically stable. The solution is prepared in cell culture grade water.
参考文献:Nature 2025年发表 Influenza A(H5N8) vaccine induces humoral and cell-mediated immunity against highly pathogenic avian influenza clade 2.3.4.4b A(H5N1) viruses in at-risk individuals

