This rabbit anti-c-Met [pYpY1234/1235] polyclonal antibody is specific to human and canine c-Met [pYpY1234/1235] and expected to react with mouse and rat (100% homology). c-Met (Hepatocyte Growth Factor receptor) is a ~ 145 kDa receptor tyrosine kinase and is required for cell migration and morphogenic differentiation. Validated application for rabbit anti-c-Met [pYpY1234/1235] polyclonal antibody is Western blot. Other applications may work but have not been tested.

• Applications: Validated application for rabbit anti-c-Met [pYpY1234/1235] polyclonal antibody is Western blot.
• Host species and isotype: The host species and isotype of the c-Met [pYpY1234/1235] polyclonal antibody is rabbit IgG.
• Reactivity: Rabbit anti-c-Met [pYpY1234/1235] polyclonal antibody detects human and canine (mouse and rat) c-Met [pYpY1234/1235].
• Product size: Rabbit anti-c-Met [pYpY1234/1235] polyclonal antibody is available in 10 blots pack size.

c-Met (Hepatocyte Growth Factor receptor) is a ~145 kDa receptor tyrosine kinase and is required for cell migration and morphogenic differentiation. c-Met receptor is activated in response to scatter factor (SF)/hepatocyte growth factor (HGF) binding. Activated c-Met undergoes dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c-Met contains three tyrosines (Tyr-x-x-x-Tyr-Tyr motif) within the activation loop of the catalytic domain, for which phosphorylation of all three (tyrosines 1230, 1234, and 1235) is required for full activation. Activation of the c-Met/HGF receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, ERK1&2, JNK1&2, PLC-γ, and STAT3).