Format

Peptide

Preparation and Storage

Reconstitute 1.0 mg of Z-FA-FMK in DMSO. We typically make a 10 mM stock solution (dissolve 1.0 mg of Z-FA-FMK in 150 µl DMSO). The final concentration of inhibitor may vary between experimental systems and therefore should be optimized by the user. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% because levels above this may cause some cellular toxicity and mask the effect of the caspase inhibitor. Store the lyophilized inhibitor at -20°C. Reconstitute the inhibitor in DMSO before use. Store the reconstituted inhibitor in small aliquots at -20°C and avoid repeated freeze-thaw cycles.

References

1. Thornberry, N.A. and Y. Lazebnik. 1998. Caspases: enemies within. Science 281:1312-1316.2. Gregoli, P.A. and M.C. Bondurant. 1999. Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors.J. Cell Physiol. 178:133-143.

Caution

Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Datasheet

RELATED PRODUCTSCat. No. 550377/69361U, Z-VAD-FMK, General Caspase InhibitorCat. No. 550378/69381U, Z-DEVD-FMK, Caspase-3 InhibitorCat. No. 550379/69391U, Z-VEID-FMK, Caspase-6 InhibitorCat. No. 550380/69401U, Z-IETD-FMK, Caspase-8 InhibitorCat. No. 550381/69411U, Z-LEHD-FMK, Caspase-9 InhibitorFigure Legend: Flow cytometric analysis of Jurkat T cells. Jurkat cells were preincubated with the following: no inhibitor, 20 µM Z-LEHD-FMK or 20 µM control inhibitor (Z-FA-FMK) for 30 min, and then either left untreated or treated with 4 µM of campthothecin for 3 hr. Following incubation, cells were collected and stained with Annexin V-PE (Cat. No. 559763/6900KK) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells approximately 42% of the cells were induced to undergo apoptosis. Cells treated with the control inhibitor Z-FA-FMK (Cat. No. 550411/69421U) showed similar results to the treated cells without inhibitor, indicating that the control inhibitor did not attenuate apoptosis.

Catalog Number

550411

Brand

BD Pharmingen™

Name

Caspase Inhibitors Negative Control, Z-FA-FMK

Size

1 mg

Regulatory Status

 RUO

Format

Purified