References

1. Thornberry, N.A. andY. Lazebnik. 1998. Caspases: enemies within. Science 281:1312-1316.2. Maccarrone, M., T. Lorenzon, M. Bari, G. Melino, and A. Finazzi-Agro. 2000. Anandamide induces apoptosis in human cells via vanilloid receptors.Evidence for a protective role of cannainoid receptors. J. Biol. Chem. 275:31938-31945.

Caution

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in plumbing.

Datasheet

RELATED PRODUCTSCat. No. 550377/69361U, Z-VAD-FMK, General Caspase InhibitorCat. No. 550378/69381U, Z-DEVD-FMK, Caspase-3 InhibitorCat. No. 550379/69391U, Z-VEID-FMK, Caspase-6 InhibitorCat. No. 550380/69401U, Z-IETD-FMK, Caspase-8 InhibitorCat. No. 550411/69421U, Z-FA-FMK, Negative Caspase InhibitorFigure Legend: Flow cytometric analysis of apoptosis in Jurkat T cells. Jurkat cells were preincubated with the following: no inhibitor, 20 µM Z-LEHD-FMK or 20 µM control inhibitor (Z-FA-FMK) for 30 min, and then either left untreated or treated with 4 µM of campthothecin for 3 hr. Following incubation, cells were collected and stained with Annexin V-PE (Cat. No. 559763/6900KK) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells approximately 42% of the cells were induced to undergo apoptosis and the use of the caspase-9 inhibitor Z-LEHD-FMK reduced the level of apoptosis to half of that level observed in treated controls. Cells treated with Z-FA-FMK (Cat. No. 550411/69421U) showed similar results to the treated cells without inhibitor, indicating that the control inhibitor did not attenuate apoptosis.

Contents

lyophilized; Reconstitute in DMSO.

Preparation and Storage

Z-LEHD-FMK has a molecular weight of 804 Daltons. The Z-LEHD-FMK inhibitoris routinely tested for its ability to inhibit apoptosis in Jurkat T cells using AnnexinV staining.Reconstitute 1.0 mg of Z-LEHD-FMK in DMSO. We typically make a 10 mM stock solution (dissolve 1.0 mg of Z-LEHD-FMK in 124 µl DMSO). The final concentration of inhibitor may vary between experimental systems and therefore should be optimized by the user. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% because levels above this may cause some cellular toxicity and mask the effect of the caspase inhibitor. Store the lyophilized inhibitor at -20°C. Reconstitute the inhibitor in DMSO before use. Store the reconstituted inhibitor in small aliquots at -20°C and avoid repeated freeze-thaw cycles.

Catalog Number

550381

Brand

BD Pharmingen™

Name

Caspase-9 Inhibitor, Z-LEHD-FMK

Size

1 mg

Regulatory Status

 RUO

Format

Purified