Preparation and Storage

Reconstitute 1.0 mg of Z-DEVD-FMK in DMSO. We typicallymake a 10 mM stock solution (dissolve 1.0 mg of Z-DEVD-FMK in 150 µl DMSO). The final concentration of inhibitor may vary between experimental systems and therefore should be optimized by the user. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% because levels above this may cause some cellular toxicity and mask the effect of the caspase inhibitor. Store the lyophilized inhibitor at -20°C. Reconstitute the inhibitor in DMSO before use. Store the reconstituted inhibitor in small aliquots at -20°C and avoid repeated freeze-thaw cycles.

Sidenote

Flow cytometric analysis of apoptosis in Jurkat T cells. Jurkat cells were preincubated with thefollowing: no inhibitor, 20 µM Z-DEVD-FMK or 20 µM control inhibitor (Z-FA-FMK) for 30 min,and then either left untreated or treated with 4 µM of campthothecin for 3 hr. Followingincubation, cells were collected and stained with Annexin V-PE (Cat. No. 559763/6900KK) toidentify cells undergoing apoptosis. The results indicate that in campthothecin treated cellsapproximately 42% of the cells were induced to undergo apoptosis and the use of the caspase-3inhibitor Z-DEVD-FMK reduced the level of apoptosis to that observed in untreated controls.Cells treated with Z-FA-FMK (Cat. No. 550411/69421U) showed similar results to the treatedcells without inhibitor, indicating that the control inhibitor did not attenuate apoptosis.

Datasheet

RELATED PRODUCTSCat. No. 550377/69361U, Z-VAD-FMK, General Caspase InhibitorCat. No. 550379/69391U, Z-VEID-FMK, Caspase-6 InhibitorCat. No. 550380/69401U, Z-IETD-FMK, Caspase-8 InhibitorCat. No. 550381/69411U, Z-LEHD-FMK, Caspase-9 InhibitorCat. No. 550411/69421U, Z-FA-FMK, Negative Caspase Inhibitor

References

1. Thornberry, N.A. and Y. Lazebnik. 1998. Caspases: enemies within. Science 281:1312-1316.2. Rickers, A., E. Brockstedt, M.Y. Mapara, A. Otto, B. Dorken, and K. Bommert. 1998. Inhibition of CPP32 blocks surface IgM-mediated apoptosis andD4-GDI cleavage in human BL60 Burkitt lymphoma cells. Eur. J. Immunol. 28:296-304.3. Schrantz, N., D.A. Blanchard, M.T. Affredou, S. Sharma, G. Leca, and A. Vazquez. 1999. Role of caspases and possible involvement of retinoblastomaprotein during TGFbeta-mediated apoptosis of human B lymphocytes. Oncogene 18:3511-3519.

Caution

Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Catalog Number

550378

Brand

BD Pharmingen™

Name

Caspase-3 Inhibitor, Z-DEVD-FMK

Size

1 mg

Regulatory Status

 RUO

Format

Purified